High pressure freezing is the most powerful cryofixation method for thicker samples and can be realized at the MPIPZ with the Leica HPM100 high pressure freezing machine.
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After high pressure freezing, samples can be processed by freeze substitution with the help of the Leica EM AFS2 automatic freeze substitution system.
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For improved resin infiltration of chemically fixed or high pressure frozen and freeze substituted samples, an evaporation-controlled automated embedding and polymerization unit (EMS poly III) is available.
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