High pressure freezing is the most powerful cryofixation method for thicker samples and can be realized at the MPIPZ with the CryoCapCell HPM Live µ high pressure freezing machine.
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After high pressure freezing, samples can be processed by freeze substitution with the help of the Leica EM AFS2 automatic freeze substitution system.
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For improved resin infiltration of chemically fixed or high pressure frozen and freeze substituted samples, an automated tissue processor (EMS Lynx II) is available with options for both vacuum and/or agitation during processing.
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